Device

Part:BBa_K2120314

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)


J23119+B0032+tetR+B0015


J23119 is strong constitutive promoter，tetR is an inhibitor which is built to repress the activity of pTet promoter. We linked two elements by medium ribosome binding site(RBS) B0032 to build this device. The expression efficiency of tetR depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None